Simpathic: Semi-automated differentiation of iPSC into NGN2 neurons

Version 1

This file is a word document that documents the semi-automated differentiation of iPSC into NGN2 neurons that took place over the course of 14 days starting on 2020/02/24 in the context of the Simpathic project. To that effect it is organised in the following table of contents:

• Materials
• Preparation of solutions, buffers and materials
  • Prepare iPSC plating medium
  • Prepare neuronal differentiation medium 1
  • Prepare neuronal differentiation medium 2
  • Prepare neuronal differentiaion medium 3
  • Prepare neuronal differentiaion medium 4
  • Prepare neuronal differentiaion medium 5
• Aim
• Protocol * Summary picture * Day 1 start of the differentiation process * Day 3 feed Diff 2 medium * Day 4 refresh 50% of the medium * Day 6 refresh 50% of the medium * Day 8 refresh 50% of the medium * Day 9 refresh 50% of the medium * Day 11 refresh 50% of the medium * Day 13 split neurons to assay plates * Day 14 end date refresh 50% of the medium
• General considerations